Characterization and analysis of complete chloroplast genome of Clausena anisum-olens (Blanco) Merrill

Abstract

In this study, the chloroplast genome (Cp) of Clausena anisum-olens ((Blanco) Merrill) was sequenced by high-throughput sequencing technology. We found that the length of the chloroplast genome of Clausena anisum-olens was 159,753 bp, and the total GC content was 38.7%, including a large single-copy (LSC) region of 87,623 bp, a small single-copy (SSC) region of 17,986 bp, and 27,072 bp pairs of reverse repeats (IRS). The Cp genome encoded 129 genes, containing 86 protein-coding, 37 tRNA, and 6 rRNA genes. Phylogenetic analysis of the genome sequence showed that Clausena anisum-olens was closely related to Micromelum minutum and Glycosmis mauritiana.

Keywords:

• Clausena anisum-olens (Blanco) Merrill
• chloroplast genome
• sequence
• gene
• phylogenetic analysis

Acknowledgments

We express our gratitude to Kunming Zhifen Biotechnology Co., Ltd for the provision of the plant material for molecular experiments.

Disclosure statement

No potential conflict of interest is reported by the author(s).

Data availability statement

The genome sequence data that support the findings of this study are openly available in the GenBank of NCBI at (https://www.ncbi.nlm.nih.gov/) under the accession no. MZ460583. The associated BioProject, SRA, and BioSample numbers are PRJNA758156, SRR15647457, and SAMN21013949, respectively.

Additional information

Funding

This work was supported by the Chongqing Science and Technology Commission [cstc2018jcyjAX0639] and the Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJCX2020046; KJQN201901222].