Next generation sequencing yields the complete mitochondrial genome of the darkfin hind Cephalopholis urodeta (Serranidae) from the Mischief Reef, South China Sea

Abstract

The complete mitochondrial genome of the Cephalopholis urodeta (Serranidae: Epinephelinae) was sequenced by next-generation sequencing method on the basis of one female specimen collected from coral reef areas at the Mischief Reef, South China Sea. The mitogenome is 16,593 bp in length, including 13 protein-coding genes, 22 tRNA genes, two rRNA genes and a D-loop region. The overall content of A + T is 55.66%, which is significantly higher than the C + G content (44.34%). Among four bases, C shows the lowest frequency (15.99%). Phylogenetic tree based on the 13 protein-coding genes was constructed for analyzing the position of C. urodeta. The results provide useful insights into further studies on population genetics and phylogenetics of groupers.

Keywords:

• Mitogenome
• next generation sequencing
• Cephalopholis urodeta
• Mischief Reef

Ethical statement

Ethics Committee approval was obtained from the Institutional Ethics Committee of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences to the commencement of the study.

CRediT authorship contribution statement

Ning Liu: Methodology, Software, Writing – original draft. Dianrong Sun: Formal analysis, Data curation. Binbin Shan: Visualization, Data curation. Yan Liu: Investigation, Writing – review and editing. Liangming Wang: Validation, Data curation. Yingbang Huang: Software, Validation. Changping Yang: Resources, Supervision, Project administration.

Acknowledgments

We thank the editor and anonymous reviewers for their valuable comments on the manuscript.

Disclosure statement

The authors report that they have no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Data availability statement

The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at (https://www.ncbi.nlm.nih.gov/) under the accession no. MZ411547. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA758836, SRR15667801, and SAMN21037224, respectively.

Additional information

Funding

This study was supported by the Hainan Natural Science Foundation [No. 319QN337], the Open Foundation of Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture [No. GDKLHSA1905], and the Guangdong Science and Technology Program Project [No. 2019B121201001].