Phylogenetic relationships and characterization of the complete chloroplast genome of Paphiopedilum ‘GZSLKY Youyou’, a hybrid of P. dianthum × P. barbigerum

Abstract

Paphiopedilum ‘GZSLKY Youyou’. is a new cultivar of Paphiopedilum with highly ornamental and horticultural value developed by crossing female parent Paphiopedilum dianthum T. Tang & F. T. Wang 1940 and male parent Paphiopedilum barbigerum T. Tang & F. T. Wang 1940. In this study, the complete chloroplast genome of the cultivar has been reconstructed from the Illumina sequencing data. The circular genome was 160,503 bp in size, containing a large single-copy region (91,582 bp), a small single-copy region (3,215 bp) and a pair of IR regions (each one 32,853 bp). The overall GC contents of the chloroplast genome was 36.20%. The chloroplast genome contained 122 genes, including 76 protein coding genes, 38 tRNA genes and 8 rRNA genes. A maximum likelihood (ML) phylogenetic tree showed that the cultivar ‘GZSLKY Youyou’ was clustered into the same clade with its parents and was closest related to P. dianthum, reflecting a maternal inheritance of chloroplasts. This complete chloroplast genome resource could be further used for genomic studies, phylogenetic analyses, and genetic engineering and breeding of the genus Paphiopedilum.

Keywords:

• Paphiopedilum
• ‘GZSLKY Youyou’
• complete chloroplast genome
• phylogenetic tree

Author contribution

P. Z. was involved in the conception and design; H. L. and H. Y. contributed the sample collection; H. Y. and P. Z. were involved in the analysis and interpretation of the data; H. L. and H. Y. contributed the drafting of the paper; H. Y. and P. Z. revised it critically for intellectual content. All authors were involved in the final approval of the version to be published. All authors agree to be accountable for all aspects of the work.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The complete chloroplast genome sequence data that support the findings of this study was submitted to GenBank under the accession number of MN315105 (https://www.ncbi.nlm.nih.gov/nuccore/MN315105.1/). The associated BioProject, SRA, and Bio-Sample numbers are PRJNA791767, SRR17318925, and SAMN24344705, respectively.

Additional information

Funding

This study was funded by the National Natural Science Foundation of China [32070372 to Peng Zhao].