Complete mitochondrial genome identification of Niwaella nigrolinea (Cypriniformes: Cobitidae)

Abstract

In this study, the complete mitochondrial genome of Niwaella nigrolinea (Cypriniformes: Cobitidae) from Zhejiang, China, was determined for the first time. We found that the sequenced length of the complete mitochondrial genome of N. nigrolinea was 16,565 bp. The genome contained 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and two non-coding regions, identical to most other vertebrates. Our phylogenetic analysis results confirmed that N. nigrolinea was close to an unclassified Cobitis sp. and the fishes of the genus Cobitis. These data would contribute to the genetic conservation genetics and stock evaluation of N. nigrolinea.

Keywords:

• Niwaella nigrolinea
• mitochondrial genome
• next-generation sequencing
• phylogeny

Authors’ contributions

Tian-Jiang Chu and Kai Liu carried out the experiments; Tian-Jiang Chu and Ning Yang assisted in sample collection; Tian-Jiang Chu assisted in sample sequencing; Kai Liu analyzed the data and wrote the manuscript. The final manuscript was reviewed and approved by all authors.

Ethical approval

Approval from the Science and Technology Bureau of China and the Department of Wildlife Administration is not required for the experiments conducted in this paper when the fish in question are neither rare nor near extinction (first- or second-class state protection level). According to Measures of Zhejiang Province on Administration of Laboratory Animals, ethical approval was not required because the approval is only necessary when researchers will use mammals.

Disclosure statement

The authors reported no potential conflict of interest.

Data availability statement

The data supporting this study’s findings are openly available in National Center for Biotechnology Information (NCBI) at https://www.ncbi.nlm.nih.gov/nuccore, reference number MZ707538. The associated BioProject, SRA, and BioSample numbers are PRJNA761196, SRR15734596, and SAMN21245545, respectively.

Additional information

Funding

This work was supported by the Science & Technology Innovation Program of Hangzhou Academy of Agricultural Sciences [2019HNCT-01, 2020HNCT-01].