The complete chloroplast genome of Primula calliantha subsp. bryophila, an ornamental alpine plant from China

Abstract

Primula calliantha subsp. bryophila (Balf. f. et Farrer) W.W. Smith and Forrest (1928) is a perennial alpine species with ornamental value. It is distributed in northwestern Yunnan and adjacent eastern Tibet of China, and northern Myanmar. Here, we sequenced and assembled complete plastid genome of P. calliantha subsp. bryophila, which is a circular molecule of 152,045 bp in length, including a large single-copy region (83,966 bp), a small single-copy region (17,663 bp), and a pair of inverted repeats (25,208 bp). The chloroplast genome contained 113 genes, including 79 protein-coding genes, four rRNA genes, and 30 tRNA genes. The phylogenetic tree based on chloroplast genomes showed the relative relationship of P. calliantha subsp. bryophila and P. calliantha, which further supports P. calliantha subsp. bryophila as a subspecies of P. calliantha in taxonomy. The complete chloroplast (cp) genome of P. calliantha subsp. bryophila provides valuable data for further phylogenetic studies of Primulaceae.

Keywords:

• Chloroplast genome
• Primula calliantha subsp. bryophila
• phylogenomic analysis

Ethics statement

Complying with ethics of experimentation: Plant samples used in this study are unprotected species. We confirm that all research is conducted in accordance with ethical guidelines and the legal requirements of the country.

Author contributions

Rui Li is mainly responsible for the data analysis and writing of the manuscript, Li Zhang is responsible for the guidance and actual participation in the related data analysis. In addition to collecting plant materials and critically revising the manuscript, Yunqi Liu and Shubao Wang also made substantial contributions to the conception of the work. Yuan Huang is responsible for supervising the project and reviewing and approving the final version of the manuscript to be released.

Disclosure statement

The authors declare that they have no competing interests.

Data availability statement

The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at [https://www.ncbi.nlm.nih.gov/] under the accession no. ON416873. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA834972, SRR19141994, and SAMN28088249, respectively.

Additional information

Funding

The research was supported by the National Natural Science Foundation of China [no. 31960050]; the Yunnan Provincial Department of Education [no. 2021Y500]; and the Major Project on Biodiversity Conservation of Ministry of Ecology and Environment.