Assembly and phylogenetic analysis of the complete mitogenome of Devario interruptus (Teleostei, Cypriniformes, Danionidae)

Abstract

The complete mitochondrial DNA genome of Devario interruptus was sequenced on the Illumina HiSeq platform and found to be 16,735 bp and included 37 genes encoding 13 proteins, 22 tRNAs, two rRNAs, and two non-coding regions. The proportion of nucleotides in mitochondrial genome was T (27.9%), C (23.7%), A (33%), G (15.4%), and the deviation of AT was 60.9%. A Maximum-Likelihood phylogenetic tree was reconstructed using the concatenated mitochondrial protein-coding genes of D. interruptus and other 18 species of fishes. Phylogenetic analysis results supported that D. interruptus was closely related to Devario shanensis. Fundamental genetic data of D. interruptus will be essential for further genetic studies.

Keywords:

• Mitochondrial genome
• Devario interruptus
• Barilius interrupta
• Barilius interruptus
• Brachydanio interrupta

Acknowledgment

The authors acknowledge the Shanghai Genesky Biotechnologies Inc., for their technical support. We thank the anonymous reviewers and editors for their valuable revision comments.

Ethical approval

The experiments were approved by the Ethics Committee for Animal Experiments of Jiangsu Agri-animal Husbandry Vocational College, and conducted following the Chinese Association for the Laboratory Animal Sciences and the Institutional Animal Care and Use Committee (IACUC) protocols.

Author contributions

Xiao Jiang Chen & Wen Zhao Liu make substantial contributions to the conception or design of the work, and drafting the paper, and Final approval of the version to be published; Lin Song & Hai Xia Liu were involved in the acquisition, analysis and interpretation of the data; the drafting of the paper, and the final approval of the version to be published. All authors agree to be accountable for all aspects of the work.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at (https://www.ncbi.nlm.nih.gov/) under the accession no. MZ853154. The associated "BioProject", "Bio-Sample" and "SRA" numbers are PRJNA769978, SAMN22187307, SRR16301567, respectively.

Additional information

Funding

This work was funded by the research project of Jiangsu Agri-animal Husbandry Vocational College under [Grant NSF2021ZR14, 11710121090]; the excellent teaching team of Jiangsu University Blue Project under [Grant 2021 − 11].