Complete mitogenome sequence of Aedes (Hulecoeteomyia) japonicus japonicus from Hawai’i Island

Abstract

We report the first complete mitogenome (Mt) sequence of Aedes japonicus japonicus (Diptera: Culicidae). The sequence was extracted from one adult from the Big Island of Hawai’i Island. The length of the Ae. japonicus japonicus Mt was 16,528bp with 78.1% AT content. Its sequence is most similar to the Mt sequence of Aedes koreicus with 90.81% sequence identity. This is the first full Mt sequence available for this species and provides important genetic resource for studying population genetics and dynamics of this important invasive mosquito species.

Keywords:

• Aedes japonicus japonicus
• arbovirus vector
• Pacific Island
• Culicidae
• invasive species

Acknowledgements

We thank UF ICBR for providing sequencing services. We appreciate the support from Gracelda Simmons from Hawai’i Department of Health toward our project and the assistance from Logan Takasaki, Tyler Gibo, Cory Shiraishi, Guy Tsutsui, and Bruce Mackey from the Hawai’i Department of Health for field collection.

Ethics statement

The study involves collection of mosquito specimen in public places (beach parks) or private properties. The verbal consent from homeowners was acquired prior to conducting collections in private properties. No permit or ethical approval is required for this study.

Author contributions

YL, CMJ, and OSA conceived experiments; CMJ and YL conducted field collection and species identification; SS, ALR-W, and VTN contributed to sequence generation; SS, ALR-W, VTN, TCC, XW, and MTR contributed to data analysis; SS, XW, MTR, and YL contributed to drafting the manuscript; all authors contributed to revision of manuscript.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article. This publication was developed under Assistance Agreement No. 84020401 awarded by the U.S. Environmental Protection Agency to Akbari. It has not been formally reviewed by EPA. The views expressed in this document are solely those of authors and do not necessarily reflect those of the Agency. EPA does not endorse any products or commercial services mentioned in this publication.

Data availability statement

The data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov/ under the accession number OP373191. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA882781, SRR21658404, and SAMN30950993, respectively.

Additional information

Funding

This study was supported by the Pacific Southwest Center of Excellence in Vector-Borne Diseases (U.S. Centers for Disease Control and Prevention Cooperative Agreement) under Grant [1U01CK000516]; the USDA National Institute of Food and Agriculture Hatch Project [1025565]; U. S. Environment Protection Agency (EPA) Science To Achieve Results (STAR) Program [84020401], the Southern IPM Center as part of USDA National Institute of Food and Agriculture Crop Protection and Pest Management Regional Coordination Program [Agreement No. 2018-70006-28884], University of Florida Department of Entomology and Nematology Matching Assistantship to Mr. Seok, University of Florida College of Agricultural and Life Sciences Dean’s Award to Ms. Nguyen, and ad hoc funds to purchase some supplies from the Hawai’i Department of Health.