Abstract
The differentiation between Neolissochilus and Acrossocheilus species based only on morphology is ambiguous; however, phylogenetic analysis using their mitogenome sequences provides conclusive results. Here, the phylogenetic position of Neolissochilus hendersoni (Herre, 1940) was determined using its mitogenome data. Total DNA from N. hendersoni was sequenced using the Illumina NovaSeq6000 platform, and annotation of mitochondrial genes was performed using MITOS2. Phylogenetic trees were constructed using the complete mitogenomes of 16 fish species. The mitogenome of N. hendersoni was found to be 16584 bp long, containing two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and three non-coding control regions. The genome showed a slight A + T bias (A + T = 56.46%). Most PCGs were found to be located on the L-strand. Results of the phylogenetic analysis showed that N. stracheyi is closely related to N. hendersoni. Our results will help to clarify the phylogenetic relationship between Neolissochilus and Acrossocheilus species.
Acknowledgments
We thank the Taylor & Francis Editing Services (https://www.tandfeditingservices.com) for its linguistic assistance during the preparation of this manuscript.
Ethical approval
This study was conducted following the guidelines and approval of the Institutional Animal Care and Use Committee at the Zhejiang Laboratory Animal Research Center and Zhejiang Ocean University. The approval number is 20210207.
Author contributions
Y.Guo and Y.C conceived the study. C.Yin and J.Wang collected the specimen. Y.Guo, Y.Wang, and C.Yin carried out the experiments and data analyses. Y.Guo wrote the manuscript with contributions from all authors. All authors approved the final manuscript and agreed to be accountable for all study aspects.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
The genome sequence data supporting this study’s findings are openly available in GenBank of NCBI at (https://www.ncbi.nlm.nih.gov/nuccore/OM202514.1/) under accession no. OM202514. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA816692, SRR18336527, and SAMN26688729.
Table 1. Difference in morphological features between Neolissochilus and Acrossocheilus.