The complete chloroplast genome of an endangered plant Artemisia borotalensis (Asteraceae) and phylogenetic analysis

Abstract

Artemisia borotalensis Poljakov is an endemic and endangered herb in China. In this study, we sequenced and analyzed the complete chloroplast genome of this species. Sequencing revealed the genome to be 151,179 bp in length, containing a large single copy region (82,862 bp), a small single copy region (18,377 bp), and a pair of inverted repeat regions (24,970 bp each). Our analyses demonstrated that it contained 133 genes, including 87 protein-coding genes, 37 transfer RNA genes, eight ribosomal RNA genes, and one pseudogene (ycf1). Furthermore, we found the genome to have an overall GC content of 37.4%. A phylogenetic analysis indicated that A. borotalensis and A. maritima clustered together as sister group to A. annua and A. fukudo clade.

Keywords:

• Artemisia borotalensis
• Asteraceae
• complete chloroplast genome
• phylogenetic analysis

Acknowledgments

We thank Meng Wei and Jiye Zheng of the Institute of Botany, Chinese Academy of Sciences, for their assistance in sample collection.

Author contributions

Ying Feng was mainly responsible for the study design. Guangzhao Jin conducted the analysis of data and drafted the manuscript. Zhibin Wen and Feng Song helped perform the analysis with constructive discussions and revised it critically for intellectual content. All authors approve the version to be published and agree to be accountable for all aspects of the work.

Regulation statement

All materials used in the study were collected in public areas of China and strictly follows the wild plant protection regulations of China. Voucher specimens were prepared and deposited at the Herbarium of the Institute of Botany, Chinese Academy of Sciences (PE).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The authors have provided all the raw data, which has been all activated. The data that support the findings of this study are openly available in GenBank at https://www.ncbi.nlm.nih.gov/ under the accession number ON964524. The associated BioProject, SRA, and BioSample numbers are PRJNA857588, SRR20363929 and SAMN29630348 respectively.

Additional information

Funding

This work was supported by the National Science Foundation of China (NSF-42271072), the Science and Technology Basic Resources Survey special (2018FY100704) and Project of National Plant Specimen Resource Center (NPSRC), E0117G1001.