Characterization of the complete mitochondrial genome sequence of Tanichthys albiventris and phylogenetic analysis

Abstract

In this study, we sequenced, annotated, and characterized the mitogenome of Tanichthys albiventris for the first time. The complete mitogenome is 16,544 bp in length, consisting of 13 protein-coding genes (PCGs), 22 transfer RNA genes, and two ribosomal RNA genes. We found nine genetic overlaps and 17 intergenic spacer regions throughout the mitogenome of T. albiventris. The A + T content of the mitogenome is 60.93%. All PCGs have the same start codon of the standard ATG, excepting for that of cytochrome c oxidase 1 (cox1) which is the GTG. A phylogenetic analysis with another 15 species of the Cyprinidae was performed using MrBayes and IQtree, based on the amino acid sequences of 13 mitochondrial PCGs. The results indicated that T. albiventris shares a close ancestry with Tanichthys albonubes.

Keywords:

• Tanichthyidae
• Tanichthys albiventris
• Tanichthys albonubes
• mitochondrial genome
• phylogenetic analysis

Acknowledgements

We appreciate anonymous reviewers for providing valuable comments on this article.

Ethical approval

Experiments were performed in accordance with the recommendations of the Ethics Committee of Xinyang Agriculture and Forestry University. These policies were enacted according to the Chinese Association for the Laboratory Animal Sciences and the Institutional Animal Care and Use Committee (IACUC) protocols.

Author contributions

Conceived and designed the experiments: Fan Li and Liangjie Zhao. Performed the experiments: Tiezhu Yang, Dongpu Li, and Wenhao Sun. Sample collection: Fan Li, Liangjie Zhao, Wenhao Sun, and Dongpu Li. Data acquisition: Chenxi Tan, Dongpu Li, and Wenhao Sun. Analyzed the data: Tiezhu Yang, Chenxi Tan, and Liangjie Zhao. Wrote the manuscript: Tiezhu Yang, Liangjie Zhao, and Fan Li. Helped to proofread the manuscript: Chenxi Tan, Liangjie Zhao, and Fan Li. All authors have read and agreed to the published version of the manuscript.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov/ under the accession no. OM687295. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA887552, SRR21820798, and SAMN31172242, respectively.

Additional information

Funding

The study was supported by the Youth Fund Project of Xinyang Agriculture and Forestry College [No. QN2021020], Foundation of Central Laboratory of Xinyang Agriculture and Forestry University [FCL202006], and Doctoral Research Initiation Project [No. 203071].