The complete chloroplast genome of Senna alata (L.) Roxb., an important medicinal plant from the Philippines

Abstract

Senna alata, a flowering shrub, is widely cultivated in the Philippines for its anti-fungal properties. Despite this, its chloroplast genome is not yet established. We assembled and annotated the complete chloroplast genome of accession from the germplasm collection of the Institute of Crop Science, University of the Philippines, Los Baños, using Illumina sequencing data. The complete cp genome was 159,176-bp long characterized by a large single copy of 88,769 bp, short single-copy of 18,301 bp and a pair of inverted repeat regions of 26,053 bp each. The overall GC content of the chloroplast genome was 36.4%. The plastome comprised 37 tRNA genes, 8 rRNA genes and 78 mRNA genes. Phylogenetic analysis showed that S. alata is closely related to S. siamea.

Keywords:

• Cassia alata
• Fabaceae
• Cassinae
• chloroplast genome
• medicinal plant

Acknowledgments

The study would like to thank the Department of Science and Technology- Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (DOST-PCAARRD) for the support. The authors would also like to thank Ronil Beliber and Arvin Medrano for cultivating the germplasm, and Eddelaine Joyce Bautista and Edna Mercado for processing the paperwork needed for the research project.

Author contributions

All authors agree to be accountable for all aspects of the work. Kristine Joyce Quiñones and Renerio Gentallan Jr. conceptualized the study, performed the experiments, analyzed the data, and wrote the paper; Michael Cedric Bartolome, Roselle Madayag, Juan Rodrigo Vera Cruz, Jessabel Magtoltol, Reneliza Cejalvo, Bartimeus Buiene Alvaran, Angeleigh Rose Cirunay and Emmanuel Bonifacio Timog planted, collected, photographed and prepared the germplasm, pressed the herbarium samples, reviewed literature, performed experiments, edited the paper; Teresita Borromeo, Nestor Altoveros, and Leah Endonela, helped conceptualized the study, validated the design of the experiment and the data presented, reviewed drafts of the paper.

Ethical approval

The collection of plant material was carried out in accordance with guide-lines provided by the authors’ institution (Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.govunder the accession no. ON653612. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA867486, SRR20980268 and SAMN30201905, respectively.

Additional information

Funding

This work was supported by the Department of Science and Technology-Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development [N9-250-21].