The mitochondrial genome of Tipula (Vestiplex) aestiva Savchenko, 1960 (Diptera: Tipulidae)

Abstract

The subgenus Vestiplex of the genus Tipula is one of the most species-rich groups in the family Tipulidae. Here, we present the first mitochondrial genome of the subgenus Vestiplex. The nearly complete mitochondrial genome of Tipula aestiva Savchenko, 1960 (Genbank accession number: OM287601) was 16083 bp in length and consisted of 13 PCGs, 22 tRNA and two rRNA genes. The phylogenetic the tree of family Tipulidae was reconstructed based on 13 PCGs sequences using the maximum likelihood method. The result strongly supported the monophyly of the family Tipulidae. The subgenus Vestiplex is indicated as the sister group of subgenera Pterelachisus and Formotipula.

Keywords:

• Tipulidae
• Tipula
• mitochondrial DNA
• phylogenetic analysis

Acknowledgements

We sincerely thank Mr. Liang Wang and Mr. Xin Li for their help during the study and Mr. Shang Gao for collecting the specimens.

Authors’ contributions

DY and YL planned and designed the research. YG performed experiments, and YG and BZ analyzed the data. YG wrote and other authors revised the manuscript. All authors have approved the manuscript for publication and agreed to be accountable for all aspects of the work.

Ethics statement

The specimen collection protocol was approved by the Ethics Committee of China Agricultural University. The field collection permission was authorized by Qilian Mountain National Nature Reserve Management Committee and the collected insect samples are non-protected species.

Disclosure statement

All authors have read and approved the final manuscript. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.

Data availability statement

The annotated mitochondrial genome described here is available in GenBank under accession OM287601. Raw data used for assembling was available in the SRA under accession SRR17634812 (https://www.ncbi.nlm.nih.gov/sra/PRJNA795034). The associated BioProject and Bio-Sample numbers are PRJNA795034 and SAMN24625269, respectively.

Additional information

Funding

The research was supported by the National Natural Science Foundation of China (No. 31772497)