The complete plastome of Glandora prostrata subsp. lusitanica (Samp.) D.C.Thomas (Boraginaceae), the first chloroplast genome belonging to the Glandora genus

Abstract

Glandora prostrata (Loisel.) D.C.Thomas (Thomas et al., 2008), besides being a common plant of western and south-western Europe and north-western Africa, is a species with a wealth of reported uses in traditional and folk medicine. The chloroplast genome of Glandora prostrata subsp. lusitanica (Samp.) D.C.Thomas (Thomas et al., 2008) isolate BPTPS049 described in this study is the first publicly available complete plastome belonging to the Glandora genus. The chloroplast genome (GenBank accession number: ON641304) is 150,041 bp in length with 37.5% GC content, displaying a quadripartite structure that contains a pair of inverted repeat regions (25,833 bp each), separated by a large (81,222 bp) and small (17,153 bp) single-copy regions. It has 131 annotated genes including 86 protein-coding genes, 37 tRNA genes, and eight rRNA genes. The phylogenetic analysis performed confirms that G. prostrata subsp. lusitanica is placed under the Boraginaceae family, which belongs to the Boraginales order. This study will contribute to conservation, phylogenetic, and evolutionary studies that comprise this traditional species relevant to the landscape of aromatic, medicinal, and condiment plants from Portugal.

Keywords:

• Glandora prostrata subsp. lusitanica
• Boraginaceae
• complete chloroplast genome
• Illumina MiSeq sequencing
• phylogenetic analysis

Author contributions

The authors had the following contribution to the paper: MTBC and FBG – conception and design; FBG – analysis and interpretation of the data; JC – collection and taxonomic identification of the studied specimen; ICL – sample processing; AA – shotgun library preparation and sequencing; FD – data management and submission; ICL, AA, FD, JC, MTBC, and FBG – manuscript drafting, critical revision for intellectual content, and final approval of the version to be published. All authors agree to be accountable for all aspects of the work herein presented.

Ethical approval

The species described and studied in this manuscript is not under legal protection status, either by national or European Union legislation, namely the 92/43/CEE Directive. A careful non-destructive collection protocol for voucher sampling was followed to guarantee the full future reproductive viability of the studied plant population.

Disclosure statement

No potential competing or conflict of interests was reported by the authors.

Data availability statement

The data supporting this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov under the accession number ON641304. The NCBI BioProject and BioSample are PRJNA848680 and SAMN28118496, respectively. The ENA BioProject and SRA for the generated reads are PRJEB55314 and ERR10047929, respectively.

Additional information

Funding

The work was conducted under the project “barcodingPTplants – DNA barcoding aromatic, medicinal, and condiment plants from Portugal” [02/SAICT/2017 n° 31074] financed by Programa Lisboa 2020, Portugal 2020, and the European Union, through the European Regional Development Fund (ERDF), and with the support of INCD funded by the Foundation for Science and Technology (FCT) and ERDF under the project 01/SAICT/2016 n° 022153. Funding from INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged, as well as iNOVA4Health [UIDB/04462/2020 and UIDP/04462/2020] and LS4FUTURE [LA/P/0087/2020], programs financially supported by FCT and the Ministério da Ciência, Tecnologia e Ensino Superior (MCTES).