Complete chloroplast genome and phylogenetic analysis of Lonicera caerulea var. edulis (Caprifoliaceae)

Abstract

Lonicera caerulea var. edulis, known as “blue honeysuckle” or “Haskap,” is a deciduous shrub that belongs to the Caprifoliaceae family. Characterized by the high cold hardiness and high quality of fruit, it has become a novel cash crop in cold regions worldwide. The lack of available chloroplast (cp) genome information limits studies of its molecular breeding and phylogeny. Here, the complete cp genome of Lonicera caerulea var. edulis was assembled and characterized for the first time. It was 155,142 bp in length with 38.43% of GC content, including 23,841 bp inverted repeat regions (IRs), an 88,737 bp large single-copy region (LSC), and an 18,723 bp small single-copy region (SSC). A total of 132 genes, including 85 protein-coding genes, 8 rRNA genes, and 39 tRNA genes were annotated. Phylogenetic analysis indicated that L. caerulea var. edulis was closely related to L. tangutica. These data and results provide a valuable resource for the development of breeding tools and genetic diversity studies for L. caerulea.

Keywords:

• Blue honeysuckle
• Haskap

Ethical statement

Appropriate permissions were granted by NEAU before sampling. The initial wild collection followed the “Regulations of the People”s Republic of China on the Protection of Wild Plants guidelines” (http://www.forestry.gov.cn) and complied with permission from the Heilongjiang Forestry and Grassland Bureau (http://lyhcyj.hlj.gov.cn/).

Authors” contributions

JWH. and CQZ. designed this research. CQZ., LJZ., XYS., QF., YZ., SLL., YL., MY., and DQ. partially participated in the experiment and data analysis. CQZ. drafted the manuscript.

Disclosure statement

No potential conflict of interest was reported by all the coauthors.

Data availability statement

The cp assembly in this study is openly available in GenBank of NCBI (https://www.ncbi.nlm.nih.gov/) under accession no. OP345475. The associated BioProject, SRA, and BioSample numbers are PRJNA870700, SRR21133392, and SAMN30383140 respectively.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China (32102332), the China Postdoctoral Science Foundation (2021MD703804), the Natural Science Foundation of Heilongjiang Province (LH2021C031) and the “Young Talents” Foundation of NEAU (20QC06).