Abstract
In this study, we determined the chloroplast genome sequence of the Austral king fern, Todea barbara (L.) Moore. The plastome of T. barbara is a typical circular form composed of 144,208 bp with two inverted repeats (IRs; 10,442 bp), a large single copy (LSC; 101,059 bp), and a small single copy (SSC; 22,265 bp). The complete sequence comprises 131 genes, namely 85 protein-coding genes, eight ribosomal RNAs, and 38 transfer RNAs. The guanine–cytosine (GC) content of the genome was found to be 39.9%. Additionally, U-to-C RNA editing sites were identified in eight genes: atpE, chlB, clpP, matK, rpl20, rpoB, rpoC1, and rpoC2. Phylogenetic analysis using 85 coding gene sequences revealed that the genera Todea and Osmunda form a clade and that the genus Osmundastrum is a sister genus to both.
Acknowledgment
The authors express their gratitude to Prof. Ki-Joong Kim of Korea University, Seoul, Korea, for providing the plant material essential for this study.
Ethical approval
Todea barbara is not included in the list of Australian threatened plants (https://www.environment.gov.au/cgi-bin/sprat/public/publicthreatenedlist.pl?wanted=flora) and was collected with permission from the Royal Botanical Garden Sydney. Research on this species, including the collection of plant materials, was conducted following the guidelines provided by Korea University and Kyoungpook National University.
Author contributions
Conceiving and designing the study, JSK and HTK; performing and analyzing data, HTK; writing the original draft, JSK and HTK; review and editing, JSK; supervision, JSK. All authors have read and agreed to the published version of the manuscript.
Disclosure statement
No potential conflict of interest was reported by the authors.
Data availability statement
The genome sequence data supporting the findings of this study are available from NCBI GenBank under accession no. OP793887 (https://www.ncbi.nlm.nih.gov/nuccore/OP793887.1/). The associated BioProject, SRA, and Bio-Sample numbers are PRJNA954512, SRR24137031, and SAMN34146952, respectively.