Complete assembly of the chloroplast genome of Amorphophallus coaetaneus S. Y. Liu & S. J. Wei 1986 (Araceae) from southwestern China

Abstract

Amorphophallus coaetaneus S. Y. Liu & S. J. Wei 1986 is a perennial herb belonging to the Araceae family in southwestern China (Guangxi and Yunnan provinces). Although this species have not been list in the red list of International Union for Conservation of Nature (IUCN), the populations are declining due to human over exploitation. To help to genetic diversity studies, we sequenced and assembled the complete chloroplast (cp) genome of A. coaetaneus (GenBank accession number of national center for biotechnology information (NCBI): OQ404947). The assembled genome revealed 175,465 bp in length with a GC content of 34.90%, including a large single-copy (LSC) region (98,561 bp), a small single-copy (SSC) region (16,504 bp) and two inverted repeat regions (IRs) (30,200 bp each). A total of 133 genes were annotated, of which 85 are protein-coding genes, 40 are tRNA genes and 8 are rRNA genes. As an output of this study, a maximum likelihood (ML) phylogenetic inference of 16 Araceae species clustered all four Amorphophallus species in one clade, and showed a relatively close relationship between the tribes Pythonieae and Colocasieae. The cp genome will serve as a basis in a more extensive molecular works covering all possible extant population of Amorphophallus, as well as conservation, breeding, and other ethnobotanical utilization of this species.

Keywords:

• Amorphophallus coaetaneus
• Araceae
• chloroplast genome
• phylogenetic analysis

Acknowledgments

The authors thank Dr. Joseph Elliot at the University of Kansas for her assistance with English language and grammatical editing of the manuscript.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Ethical approval

This study includes no human, animal, or endangered plant samples, and the sample was collected legally following guidelines provided by the authors’ institution and national or international regulations.

Author contributions

Yong Gao performed the molecular experiment. Yin Si and Kun Dong analyzed the data. Penghui Xiao and Weijia Wu collected the sample. Yong Gao wrote the manuscript. All authors have revised and approved the final version of this manuscript.

Data availability statement

The chloroplast genome of Amorphophallus coaetaneus was deposited to the national center for biotechnology information (NCBI) (Bethesda, MD 20894, USA) GenBank database (https://www.ncbi.nlm.nih.gov/) under the accession number OQ404947. The associated BioProject, Bio-Sample, and SRA numbers are PRJNA943846, SAMN33734031, and SRR23825299, respectively.

Additional information

Funding

This work was supported by the Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities [202101BA070001-011].