Characterization of the complete chloroplast genome of a chinese endangered species Cymbidium wenshanense Y. S. Wu et F. Y. Liu

Abstract

Cymbidium wenshanense Y. S. Wu et F. Y. Liu is of significant ornamental and breeding value. In this study, Illumina high-throughput sequencing was used to sequence and analyze the complete chloroplast genome of C. wenshanense, and the phylogenetic relationships between this and other Orchidaceae species were established. The whole chloroplast genome was 156,292 bp in length and contained 84 mRNA genes, 45 tRNA genes, and 4 rRNA genes. The phylogenetic tree of 11 Orchidaceae species revealed C. wenshanense to be most closely related to Cymbidium mastersii.

Keywords:

• cymbidium wenshanense
• chloroplast genome
• phylogenetic analysis

Acknowledgments

The authors acknowledge the Wuhan Genoseq Technology Inc., for their technical support. The authors thank International Science Editing (http://www.internationalscienceediting.com) for editing this manuscript, and the anonymous reviewers and editors for their valuable comments.

Ethical approval

Data collection was compiled according to the International Union for Conservation of Nature (IUCN) policies on research involving species at risk of extinction (see Guidelines for appropriate uses of IUCN Red list data), the Convention on Biological Diversity and the Convention on the Trade in Endangered Species of Wild Fauna and Flora.

Author contribution

X. W., Y. W. and Z. L. were involved in the conception and design; L. C. and Z. L. contributed the sample collection; X. W., Z. Z. and L.C. performed the analysis and interpretation of the data; X. W., L. C. and Z. Z. contributed the drafting of the paper; Z. L. and Y. W. revised it critically for intellectual content. All authors were involved in the final approval of the version to be published. All authors agree to be accountable for all aspects of the work.

Disclosure statement

No potential conflict of interest was reported by the authors.

Data availability statement

The data that support the findings of this study are openly available from the NCBI GenBank at https://www.ncbi.nlm.nih.gov, reference number ON782579.

The associated BioProject, SRA and Bio-Sample numbers are PRJNA764364, SRR15959874 and SAMN21500377, respectively.

Additional information

Funding

This work was supported by the Project for Innovation Team of Yunnan Province [202105AE160012], Yunnan Province Major Project of China [grant number 202102AE090052].