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Abstract
The genus Coelastrum Nägeli (Sphaeropleales; Scenedesmaceae) is a diverse genus of green algae with potential biotechnical applications. A sound understanding of its phylogeny will be a useful tool for predicting the distribution of traits that may enhance its utility, and may lead to a better understanding of its evolution and ecology. Here we present the plastome of Coelastrum microporum. Our exemplar was isolated from Gull Lake, Michigan and the complete plastome as assembled was 169,961 bp in length. The plastome contained 104 genes of which 68 were protein-coding genes (CDSs), 27 tRNA genes and three rRNA genes. The GC content of the plastome was 31.2%. The maximum likelihood phylogeny suggested that C. microporum was the sister group to a clade of single exemplars of three other genera in the Scenedesmaceae (Tetradesmus, Pectinodesmus and Coelastrella).
Authors’ contributions
Edward Theriot, Joshua Cooper, Chanhee Lee, and Trisha Spanbauer conceived and designed the experiments. Edward Theriot acquired and isolated the alga. Chanhee Lee, and Ana Salim grew the algae and extracted DNA; Chanhee Lee, Ana Salim, Chaehee Lee, and Francesca Moroni made substantial contributions to the acquisition and analysis of data; Chanhee Lee and Edward Theriot contributed substantially to authorship; all authors contributed to revision of the manuscript, and all approved the final version to be published. All authors agree to be accountable for all aspects of the work.
Disclosure statement
The authors report that there is no conflict of interest involved.
Geolocation
Gull Lake, Kalamazoo County, Michigan. 42.403061 N; 85.414341 W.
Research statement
Phytoplankton sampling and cell isolation was done following all local, state, and US Federal regulations, in the publicly accessible Gull Lake. To our knowledge there is no convention on ethical treatment of microalgae.
Data availability statement
The genome sequence data that supported the findings of this study are openly available in GenBank of NCBI at (https://www.ncbi.nlm.nih.gov/) under the accession no. NC_068582. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA909268, PRJNA909268, and SAMN32062636, respectively. The strain used is available from the UTEX Culture Collection of Algae (https://utex.org, Dr. David Nobles, Curator and Director: [email protected]) as Coelastrum microporum UTEX LB 3178.