The complete chloroplast genome sequence and phylogenetic analysis of Heritiera fomes Buch.-Ham. (Malvales: Sterculiaceae)

Abstract

Heritiera fomes Buch.-Ham. (1800) is a species of mangrove in the family Malvaceae, widely distributed in the Indo-Pacific and listed as ‘endangered’ (EN) on the International Union for Conservation of Nature’s (IUCN) red list. We reported the complete chloroplast genome sequence of H. fomes. The genome was 168,521 bp in length and included two inverted repeats (IRs) of 34,496 bp, separated by a large single-copy (LSC) region of 88,604 bp and a small single-copy (SSC) region of 10,925 bp, respectively. The genome contained 87 protein-coding genes (PCGs), 8 rRNA genes, and 37 tRNA genes. The maximum-likelihood (ML) phylogenetic tree suggested that H. fomes is closely related to Heritiera angustata and Heritiera parvifolia with relatively high support bootstrap values of 86% and 100% with other species (Heritiera littoralis and Heritiera javanica), suggesting a relatively close genetic relationship between the five Heritiera plants. The chloroplast genome sequence provided a useful resource for conservation genetics studies of H. fomes and for phylogenetic studies of Heritiera.

Keywords:

• Heritiera fomes
• Malvaceae species
• chloroplast genome (cpDNA)

Author contributions

TY, WP, and ST designed the research study and obtained the funding. SY, CN, and PP performed laboratory work (sample collection, DNA extraction, library construction, and sequencing). WK and CS performed bioinformatics analyses. TY wrote and revised the manuscript, and all authors reviewed it.

Ethical approval

Research and collection of plant material were conducted according to the guidelines for the implementation of the ‘IUCN Policy Statement on Research Involving Species at Risk of Extinction,’ with special reference to the Scientific Collecting of Threatened Species (July 2022) provided by the Mangrove Forest Research Center, the Department of Marine and Coastal Resources. Permission was granted by the Department of Marine and Coastal Resources (Ministry of Natural Resources and Environment, Thailand) under grant number P19-52261 to carry out research on the species.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at (https://www.ncbi.nlm.nih.gov/) under the accession no. OM022247. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA783468, SRR17659486, and SAMN23430239, respectively.

Additional information

Funding

This study was supported by a grant from the National Science and Technology Development Agency (NSTDA), Thailand, under grant number [P19-52261].