Abstract
In this study, we assembled the complete chloroplast (cp) genome of Cynanchum acutum subsp. sibiricum using high-throughput Illumina sequencing reads. The resulting chloroplast genome assembly displayed a typical quadripartite structure with a total length of 158,283 bp, which contained a pair of inverted repeat regions (IRs) of 24,459 bp. These two IRs were separated by a large single-copy region (LSC) and a small single-copy region (SSC) of 89,424 bp and 19,941 bp in length, respectively. The C. acutum subsp. sibiricum cp genome contained 130 genes, and its overall GC content was 37.87%. Phylogenetic analysis among C. acutum subsp. sibiricum and nine other Cynanchum species demonstrated that C. acutum subsp. sibiricum was closely related to C. chinense. The C. acutum subsp. sibiricum cp genome presented in this study lays a good foundation for further genetic and genomic studies of the Cynanchum as well as Apocynaceae.
Acknowledgments
The authors are really grateful for the access to raw genome data from public database.
Author contributions
Lei Zhang conceived and supervised the project. Erdong Zhang, Yi Liu, Yan Wang and Xuedan Zhang analyzed the data and wrote the manuscript. All of the authors have read and approved the final manuscript and have agreed to be accountable for all aspects of the work.
Disclosure statement
The coauthors do not have any conflict of interest to declare. The authors alone are responsible for the content and composition of the paper. Additionally, fresh and mature leaves from an adult plant of C. acutum subsp. sibiricum were taken with the permission of the community management department.
Data availability statement
The sequenced data supporting the findings of this study are openly available in NCBI (https://www.ncbi.nlm.nih.gov/) under the accession no. OQ390041. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA930674, SRR23341524, and SAMN33018279, respectively.