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Abstract
As one of the top 10 famous flowers in Chinese tradition, Rhododendron przewalskii subsp. przewalskii known as ‘beauty in flowers,’ which has high ornamental and medicinal value. The complete chloroplast (cp) genome of R. przewalskii subsp. przewalskii was determined in this study. The complete chloroplast genome of R. przewalskii subsp. przewalskii was 201,233 bp in length and contained a large single-copy region (LSC, 108,077 bp), and a small single-copy region (SSC, 2624 bp) and a pair of inverted repeat regions (IRa and IRb, 45,266 bp). A total of 142 functional genes were observed in this cp genome, including 91 protein-coding genes (PCGs), 43 transfer RNA genes (tRNAs), and eight ribosomal RNA genes (rRNAs). The R. przewalskii subsp. przewalskii cp genome has an A + T content of 64.06% and presents a positive AT-skew (0.53%) and a negative GC-skew (−1.56%). The maximum likelihood phylogenetic analysis based on the concatenated nucleotide sequences of 13 PCGs strongly supported the monophyletic relationship of R. przewalskii subsp. przewalskii the clade of R. henanense subsp. lingbaoense. This study provides genomic evidence for the vegetation classification of Rhododendron.
Author contributions
Tao Wang conceived, designed, and completed the manuscript. Yuling Li and Xiuzhang Li performed field collection and identification of plant material. Zhengfei Cao and Hui He assembled and annotated the chloroplast genome lineage sequences. Chuyu Tang and Jianbo Chen performed a phylogenetic analysis and visualized the maps. All authors discussed the results, critiqued the manuscript, and validated the current version of the manuscript.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
Supplementary Figure 2(A) is the sequencing depth map and coverage map and Supplementary Figure 2(B) is the black-white arrow shows the cis-spliced genes (left), and the black-grey-white arrow shows trans-spliced genes (rps12) (right), which the authors have placed in the Supplementary.
The genome sequence data that support the findings of this study are openly available in GenBank of NCBI under the accession(https://www.ncbi.nlm.nih.gov/nuccore/OQ054014.1/). The associated BioProject, SRA, and Bio-Sample numbers are PRJNA947480, SRR23938567, and SAMN33850058, respectively.