Characteristics and phylogenetic analysis of the complete mitochondrial genome of Glyptothorax pallozonus (Siluriformes, Sisoridae)

Abstract

Glyptothorax pallozonus Lin, 1934 is a small benthic fish belonging to the Sisoridae family that is distributed in the Dongjiang and Rongjiang Rivers of China. In the present study, we sequenced and characterized the complete mitochondrial genome of G. pallozonus for the first time. The complete mitogenome of G. pallozonus is 16,542 bp in length and includes 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNA (rRNAs), and a control region (CR). The mitogenome architecture was identical to that of other teleosts. Maximum likelihood (ML) phylogenetic analysis strongly supported the monophyly of Glyptothorax, which contains two clades. These results advance our understanding of the molecular phylogeny of the genus Glyptothorax.

Keywords:

• Glyptothorax pallozonus
• mitochondrial genome
• Sisorida
• phylogenetic analysis

Acknowledgments

We are grateful to Shujie Liu, Binbin Zhan and Huiwen Xiao for help in field assistance and the species reference image.

Author contributions

QFG and ZLQ conceived this study; QFG and YS conducted the experiments, LAP and WQ analyzed the data; QFG wrote the drafting of the paper; QFG and ZLQ revised it critically. All authors contributed to the article and approved the submitted version.

Data availability statement

The mitochondrial genome sequence is available on GenBank of NCBI at www.ncbi.nlm.nih.gov with the accession number of OP723308.

Disclosure statement

No potential conflict of interest was reported by the authors.

Ethical approval

The species used in this study is not protected under CITES or wildlife laws in China, and its status is data deficient by the IUCN. Therefore, no specific permission or license is required for research sampling according to Regulations of the People’s Republic of China. The live samples were obtained in accordance with the guidelines of the animal care and Ethical Committee of Freshwater Fisheries Research Institute of Jiangsu Province and Animal Experiments of Jiangsu Agri-animal Husbandry Vocational College.

Additional information

Funding

This study was supported by the Earmarked fund for Jiangsu Agricultural Industry Technology System [JATS(2022)517] and the research project of Jiangsu Agri-Animal Husbandry Vocational College (NSF2023TC05).