Characterization of the complete mitochondrial genome and phylogenetic analysis of bean thrips Megalurothrips usitatus (Bagnall, 1913) (Thysanoptera: thripidae)

Abstract

Megalurothrips usitatus (Bagnall, 1913) (Thysanoptera: Thripidae) is a widely distributed pest in Asia that primarily affects the production of snap beans and cowpea. The complete mitochondrial genome of Megalurothrips usitatus has been sequenced and annotated in this study, which is 17,209 bp long and contains 13 protein-coding genes (PCGs), two rRNAs, and 22 tRNA genes. Most of the protein-coding genes (PCGs) start with ATG except ND4 using TTG. Meanwhile, eight PCGs stop with TAA, four PCGs have an incomplete stop codon, and the gene Cytb ends with TAG. Phylogenetic analysis showed that M. usitatus is closely related to Frankliniella intonsa and F. occidentalis, providing a basis for the study of the mitochondrial evolution of Thripinae.

Keywords:

• Mitogenome
• protein-coding genes
• transfer RNA
• ribosomal RNA
• phylogenetic position

Author contributions

J-H L planned and designed the research. P L collected the insect materials, X-M L performed experiments, D-C C and X-M L analyzed the data. X-M L and P L wrote the manuscript.

Ethics approval and consent to participate

The material used in this study is widely distributed in southern regions of China and does not belong to the IUCN Red List. No specific permits were required for animal collection. The study did not require ethical approval or consent, as no endangered or protected animal species were involved.

Disclosure statement

All authors have read and approved the final manuscript. No conflict of interest was reported by the authors.

Data availability statement

The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov/ under the accession no. ON815612. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA935568, SRP423475 and SAMN33321779, respectively.

Additional information

Funding

This study provides the complete mitochondrial genome of thrips collected from Haikou (E110°11′, N20°02′) in Hainan, China. This study was supported by Hainan Provincial Natural Science Foundation of China under Grant (number 323MS018); the Startup Foundation for Introduced Talents of Hainan University under Grant (KYQD-ZR-1962); and Hainan Academician Innovation Platform research project (YSPTZX202021).