Complete mitochondrial genome sequence of Lanmaoa macrocarpa (Boletales, Basidiomycota)

Abstract

Lanmaoa macrocarpa is a boletoid mushroom from the family Boletaceae and was named after its relatively larger basidiocarp and bluish color change when bruised. At present, its mitochondrial genome and phylogenetic relationships with other boletes remain unexplored. In this study, we sequenced the mitochondrial genome of L. macrocarpa using next-generation sequencing technology and found that its mitochondrial genome, a circular DNA molecule of 38,139 bp, comprised 15 core protein-coding genes, 26 transfer RNA genes and two ribosomal RNA genes. The mitochondrial genome had a base composition of A (37.05%), C (12.08%), G (11.42%) and T (39.45%) with a GC content of 23.50%. A phylogenetic tree based on 20 mitochondrial genomes was constructed, which revealed the phylogenetic relationships of this species with related boletes for the first time.

Keywords:

• Basidiomycota
• bolete
• mitochondrial genome
• phylogenetic analysis

Author contributions

YTZ and LLC collected the samples, performed the analysis and interpretation of the data, and prepared the manuscript. KZ devised the project, revised the manuscript critically and approved the final version to be published. All authors discussed and critically revised the results and contributed to the final version of the manuscript.

Disclosure statement

The authors have declared that no competing interests exist.

Data availability statement

The mitochondrial genome data is available with the accession number of OR004349 in the GenBank of NCBI (https://www.ncbi.nlm.nih.gov/). And the associated Bioproject, SRA, Bio-sample numbers are PRJNA957944, SRR24299300 and SAMN34273906, respectively.

Ethical approval

No ethical issues were involved in this study. The collection of the mushroom was legal and reasonable. Information of the voucher specimen and who identified it were included in the manuscript.

Additional information

Funding

This work was supported by the Funds of the National Natural Science Foundation of China [31760007] and the innovation training programs for undergraduates in Jiangxi Science & Technology Normal University [20231704119].