The complete chloroplast genome of Phyla nodiflora (Linnaeus) Greene (1899) from the Verbenaceae family and its phylogenetic analysis

Abstract

Phyla nodiflora (Linnaeus) Greene (1899) is a perennial creeping herb belonging to the family Verbenaceae. It has numerous pharmacological properties, including anti-dandruff, anti-inflammatory, anti-oxidant, anti-melanogenesis, anti-hypertensive, and anti-hyperuricemic properties. We generated the complete chloroplast genome sequence of P. nodiflora using Illumina paired-end sequencing data. The P. nodiflora chloroplast genome is 154,341 bp in length, containing a large single copy (LSC) region of 85,185 bp and a small single copy (SSC) region of 17,222 bp, separated by a pair of inverted repeats (IRs) of 25,967 bp. The genome contained 128 genes, including 86 protein-coding, 34 tRNA, and eight rRNA genes. Six genes had one intron, one gene had two introns, and the others did not have an intron. Overall GC content of the chloroplast genome was 39%, while those of LSC, SSC, and IR regions were 38.2%, 33.7%, and 44%, respectively. Phylogenetic analysis of the chloroplast genome revealed that P. nodiflora is closely related to the other species from Verbenaceae.

Keywords:

• Chloroplast genome sequence
• Lippia nodiflora
• phylogeny tree
• Poduthalai
• Turkey tangle frog fruit

Ethical approval

No permissions were required for the sample collection of Phyla nodiflora L. because it is widely distributed in the wastelands and roadsides in tropical regions. The plant species was collected from Guduvanchery, Chengalpattu District, Tamil Nadu, India (GPS coordinates: 12°51′16.1″N 80°03′49.4″E).

Author contributions

Ray Sharmishtha, Tanuja, and R. Balaji collected the specimen material, conducted the experiment, analyzed the sequence data, and drafted the paper. M. Parani contributed to the conception and design of this work. All the authors carefully read, revised, and approved the final manuscript to be published. We thank Dr. Senthilkumar Umapathy for his help in the authentication of plant material.

Disclosure statement

The authors reported no potential competing interests.

Data availability statement

The genome sequence data supporting the findings of this study are available in NCBI under the accession number OQ673174 (https://www.ncbi.nlm.nih.gov/nuccore/OQ673174.1). The associated next-generation sequencing data files are available from the BioProject, Bio-Sample, and SRA submission under the accession numbers PRJNA945246, SAMN33776799, and SRR23875832, respectively.

Additional information

Funding

We acknowledge the funding from the SRM-DBT Partnership Platform for Contemporary Research Services and Skill Development in Advanced Life Sciences Technologies [Grant No. BT/PR12987/INF/22/205/2015] and SRM Institute of Science and Technology for providing research and computation facilities.