Complete mitochondrial genome and draft chloroplastic genome of Haslea ostrearia (Simonsen 1974)

Abstract

The first completed, circular mitochondrial genome and the first draft, linear chloroplastic genome of the blue diatom Haslea ostrearia (Simonsen 1974, Naviculaceae, Bacillariophyceae) were assembled from Illumina and PacBio sequencing. The mitochondrial genome was composed of 38,696 bases and contained 64 genes, including 31 protein-coding genes (CDS), 2 ribosomal RNA (rRNA) genes and 23 transfer RNA (tRNA) genes. For the chloroplast, the genome was composed of 130,200 bases with 169 genes (131 CDS, 6 rRNA genes, 31 tRNA genes, and 1 transfer messenger RNA gene). Phylogenetic trees, using the maximum-likehood method and partial genes currently available for Haslea ostrearia and other diatom species, suggested the proximity of all the Haslea ostrearia strains/isolates and the possibility of using these genomes as future references.

Keywords:

• Haslea ostearia
• diatom
• blue microalgae
• chloroplast
• mitochondrion

Acknowledgement

We express our sincere thanks to the Genotoul platform (Toulouse, France) for all sequencing data.

Author contribution

A.P, H.G, M.F were involved in the conception and design; H.G, M.F contributed to the DNA extraction; A.P, F.S performed the analysis and interpretation of the data; A.P, F.S, B.C, N.C contributed the drafting of the paper; H.G, M.F, JL.M revised it critically for intellectual content; N.C and JL.M found the financing. All authors were involved in the final approval of the version to be published. All authors agree to be accountable for all aspects of the work.

Disclosure statement

No potential conflict of interest was reported by the authors.

Data availability statement

The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov/ under the accession no. ON548138 and ON548139. The associated BioProject, SRA and Bio-Sample numbers are PRJNA843895, SRR19450090/SRR19450089, and SAMN28772203 respectively.

Additional information

Funding

This research was supported by a Ph.D grant for A.P co-funded by Region Pays de la Loire (Lancom project) and Le Mans University. M.F. benefited from the Horizon 2020 Research and Innovation Program GHaNA (The Genus Haslea, New marine resources for blue biotechnology and Aquaculture, grant agreement No 734708/GHANA/H2020-MSCA-RISE-2016).