The complete mitochondrial genome of Goniurosaurus varius (Squamata: Eublepharidae)

Abstract

The complete mitochondrial genome sequence of Goniurosaurus varius is 17778 bp in length (GenBank accession number OQ992199), containing 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes. The gene order and orientation are identical to those of other Eublepharidae species in the GenBank database. Seven protein-coding genes (COX2, COX3, ND1, ND2, ND3, ND4 and CYTB) exhibit incomplete stop codon ‘T.’ Phylogenetic analysis revealed the monophyly of Goniurosaurus and Eublepharidae and suggested that G. varius is closely related to the lineage composed of G. luii and G. liboensis. Distinct from other published Eublepharidae species, G. varius contains an extra non-coding region between tRNA-Thr and tRNA-Pro, which may be formed by gene rearrangements. The complete mitochondrial genome will be helpful for further studies on the population genetics of this species and phylogenetic analyses of Eublepharidae.

Keywords:

• Goniurosaurus varius
• Illumina sequencing
• mitogenome
• phylogenetic analysis

Acknowledgement

We want to express our gratitude to the Institute of Herpetology, Shenyang Normal University, for the facilities and support of this research project.

Author contributions

YZ, PL and ZZ designed and conceived this work; LD, ZL, Longming Fu collected the samples; LD, LX and HC performed the DNA isolation; ZL, Lin Feng and SY performed the mtgenome assembly; Longming Fu, Lin Feng and SY performed the phylogenetic analysis; ZZ and YZ wrote the first version of the manuscript; and PL and ZZ carried out the investigation. All the authors have read, revised and approved the final manuscript.

Ethics statement

The Committee on the Ethics of Animal Experiments of the College of Life Sciences, Shenyang Normal University, permitted and approved the study (SYNU-20220203) to comply with the International Union for Conservation of Nature (IUCN) policies for research involving species at risk of extinction.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The genome sequence data that support the findings of this study are openly available in GenBank of the NCBI at https://www.ncbi.nlm.nih.gov/ under accession no. OQ992199. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA995366, SRX21042629, and SAMN36468329, respectively.

Additional information

Funding

This work was supported financially by the Research Fund for the Research and Development Project of Liaoning Forestry and Grassland Bureau [number LLC202319], the Educational Commission of Liaoning Province of China [grant number LQN201904] and the Natural Science Foundation of Liaoning Province of China [grant number 2020-BS-148].