The complete chloroplast genome of Chloranthus nervosus Collett ex Hemsl. 1890 (Chloranthaceae)

Abstract

In this study, we sequenced and assembled the complete chloroplast genome of Chloranthus nervosus Collett ex Hemsl. 1890. The total length of the complete chloroplast sequence was found to be 158,002 bp. It consisted of a large single-copy (LSC) region of 87,127 bp, a small single-copy (SSC) region of 18,541 bp, and a pair of inverted repeat (IR) regions, each with a length of 26,167 bp. The overall GC content of the complete chloroplast genome was 38.9%, with the LSC region, SSC region, and IR regions exhibiting GC contents of 37.4%, 34.1%, and 43.1%, respectively. The annotation of the chloroplast genome revealed a total of 131 genes, comprising 86 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis revealed that the seven sampled species of Chloranthus were divided into two clades. Within the clade characterized by long filamentous anther connectives, C. nervosus showed the closest relation to C. japonicus. These findings validated the previous preliminary results on the phylogenetic relationships of the seven species of Chloranthus with strong support.

Keywords:

• Chloranthus nervosus
• chloroplast genome
• phylogenetic analysis

Ethical approval

The C. nervosus specimens used in this study are not endangered, protected, or privately owned. Therefore, no permission was necessary for collecting plant materials in this study. We confirm that the research adheres to ethical guidelines and local legislation.

Author contributions

Shu-Ting Yao: analyzed data and drafted the manuscript. Yong-Bin Lu: collected materials, conducted species identification, and facilitated the sample preparation for sequencing. Zhan-Jiang Zhang and Cui Li: provided financial program support and helped to revise the manuscript. Peng-Fei Wang and Xin-Mei Qin: designed the study, provided guidance for the analysis, and revised the manuscript. All authors have reviewed and approved the final manuscript for publication.

Disclosure statement

No potential conflicts of interest are reported by the authors.

Data availability statement

The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at (https://www.ncbi.nlm.nih.gov/) under accession no. OR198055. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA990663, SRR25131669, and SAMN36271954, respectively.

Additional information

Funding

This study was supported by the Guangxi Natural Science Foundation Project [2021GXNSFAA196049; 2020GXNSFAA159151] and Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain [22-035-26].