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Abstract
The authors sequenced the complete mitochondrial (mt) genomes of the band-legged ground cricket (Dianemobius fascipes nigrofasciatus Matsumura, 1904) and a temperate form of the lawn ground cricket (Polionemobius taprobanensis Walker, 1869), collected in Japan. The length of the mt genome sequences was 15,354 bp in D. fascipes nigrofasciatus and 16,063 bp in P. taprobanensis. Annotation of the mt genome sequences revealed 13 protein-coding genes, two rRNA genes, and 22 tRNA genes. The orientation of the genes was the same as in other Grylloidea species, and the order was the same as in other Trigonidiidae species. In our phylogenetic analysis, D. fascipes nigrofasciatus formed a clade with D. fascipes collected in China, and the temperate form of P. taprobanensis formed a clade with P. taprobanensis collected in China. Comparison of the numbers of positions with different amino acid residues encoded by the protein-coding genes implied the separate species status of each member of each of the two pairs of ground crickets. The mt genome sequences of D. fascipes nigrofasciatus and P. taprobanensis will contribute to phylogenetic and taxonomic studies of the Trigonidiidae.
Acknowledgments
The authors thank Prof. Masato Kiyomoto (Tateyama Marine Laboratory, Institute of Marine and Coastal Research, Ochanomizu University) for his support in sampling the crickets used in this study. We also thank the hosts of the website orthoptera.jp at https://www.orthoptera-jp.com/ for sharing the literature on cricket classification.
Ethical approval
This research does not involve ethical research. Insects are invertebrates, and there are no ethics involved in using them in experiments.
Author contributions
KM, KK, AO, TA, KY, and TS conceived and planned the study. KM, KK, and RS performed sample collection, wet processes, and bioinformatics analyses. KS supported the bioinformatics analyses. All the authors wrote the manuscript.
Disclosure statement
No potential conflict of interest was reported by the authors.
Data availability statement
The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov/under the accession nos. OP854596 (D. fascipes nigrofasciatus) and OP854597 (temperate form of P. taprobanensis). The associated BioProject, SRA, and Bio-Sample numbers are PRJNA926495 (D. fascipes nigrofasciatus and temperate form of P. taprobanensis), SRX19142015 (D. fascipes nigrofasciatus) and SRX19142016 (temperate form of P. taprobanensis), and SAMN32875128 (D. fascipes nigrofasciatus) and SAMN32875129 (temperate form of P. taprobanensis), respectively.