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Abstract
Huperzia arctica (Tolm.) Sipliv. 1973 is a lycophyte species belonging to the Lycopodiaceae family, which is widely distributed in the Arctic region of Svalbard, Norway. To determine its taxonomic position, we sequenced the chloroplast genome of H. arctica. The complete chloroplast genome of H. arctica is 153,956 bp in length with 122 annotated genes, including 87 protein-coding genes, 31 tRNA genes, and 4 rRNA genes. To evaluate its evolutionary position, we performed phylogenetic analysis using 36 conserved orthologous protein-coding gene sequences from the chloroplast genomes of H. arctica and publicly available data from other Lycopodiaceae. H. arctica formed a monophyletic group with four other Huperzia species: H. lucidula, H. serrata, H. crispata, and H. javanica. However, it appeared as a separate species with a highly supported bootstrap value.
Ethical approval
The materials used in this study are not included in the IUCN Red List of Threatened Species, and the sampling site is not located in any protected area. The field study was conducted with the permission granted by the Governor of Svalbard (RIS-ID 11964).
Authors’ contributions
YS and HL planned experiments. YH and YKL sampled plants. YH and HL extracted DNA, assembled the chloroplast genome and carried out the phylogenetic analysis. YH, YKL, YS, and HL wrote the manuscript. All authors have reviewed and approved the manuscript.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov under the accession no. OP714151. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA941097, SRP425714, and SAMN33589810, respectively.