Characterization of chloroplast genome and phylogenetic analysis of the Elaeagnus hybrid variety E. ‘viridis’ (Elaeagnaceae)

Abstract

Elaeagnus ‘viridis’, an artificial hybrid of E. macrophylla (♂) Thunb. (1784) × E. pungens (♀) Thunb. (1784), is known for its economic and ecological value. In this study, we sequenced and assembled the whole chloroplast (cp) genome of E. ‘viridis’. The results showed that its cp genome was 152,284 bp long, showing a typical quadripartite structure and containing a large single-copy region (LSC, 82,299 bp), a small single-copy region (SSC, 18,239 bp), and a pair of inverted repeats (IRs, 51,746 bp). The cp genome contains 132 genes, including 86 protein-coding genes (PCGs), 38 tRNA genes, and 8 rRNA genes. Phylogenetic analysis based on 66 common PCGs revealed that E. ‘viridis’ is most closely related to its maternal parent E. pungens. The chloroplast genomic information reported in this study will shed some useful light for further genetic studies in the genus Elaeagnus.

Keywords:

• Elaeagnus ‘viridis’
• complete chloroplast genome
• phylogenetic analysis

Authors contributions

Naiwei Li was mainly responsible for the design of the experiment; Fan Zhang and Xinran Chong were mainly responsible for the writing and revising of the paper; Naiwei Li and Hong Chen had an important role in the introduction and breeding of the plant materials; Yanwei Zhou was responsible for plant growth management and sample collection; Ting Zhou participated in the sample preparation work and was responsible for genomic DNA extraction; Fan Zhang and Yanwei Zhou analyzed and interpreted the data; All authors are accountable for all aspects of the work and approved the final version of the paper.

Ethical approval

The plant material (Elaeagnus pungens Thunb.) used in this study is not a protected plant, therefore, no permission or approval was required. The collection of the plant samples was conducted according to the guidelines provided by Nanjing botanical garden Memorial Sun Yat-sen and did not harm the growth of plants.

Disclosure statement

No potential conflict of interest was reported by the authors.

Data availability statement

The data of this study are available in GenBank of NCBI (https://www.ncbi.nlm.nih.gov) under accession No. OM935758. The associated BioProject, SRA, and BioSample numbers are PRJNA813133, SRR18238883, and SAMN26449928, respectively.

Additional information

Funding

This work was supported by the Public Science and Technology Research Funds Project of Oceans [grant number: 201505023], the Natural Science Foundation of Jiangsu Province [grant no. BK20201244], and the Natural Science Foundation of Jiangsu Province [grant no. BK20210163]