First record of the complete mitochondrial genome of Botyodes diniasalis (Walker, 1859) (Lepidoptera: Crambidae)

Abstract

We performed the first sequencing of the complete mitogenome of Botyodes diniasalis by high-throughput sequencing. A circular DNA molecule of 15,219 bp in size, encoding 2 rRNAs, 22 tRNAs, and 13 PCGs, contains a non-coding AT-rich region. The overall nucleotide composition of the genome is A (39.5%), T (41.3%), C (11.3%), and G (7.8%). Phylogenetic analysis based on mitogenomic data suggest that the species B. diniasalis has a close evolutionary relationship with B. principalis in Margaroniini. The complete mitogenome of B. diniasalis will serve as a valuable resource for future studies on evolution, taxonomy, genetic conservation, and utilization of Botyodes.

Keywords:

• Botyodes diniasalis
• margaroniini
• mitogenome
• phylogenetic analysis
• Illumina sequencing

Authors’ contributions

DX, DLF, YXJ, LJX, XHY, ML and CQ wrote the manuscript. DX, YLY, WX and XX critically revised the paper’s content and collected specimens of B. diniasalis. DX, LJX, XHY and YXJ photographed and processed the specimens experimentally. DX, ML, and CQ acquired and analyzed the data. DLF, YXJ, and XX interpreted and uploaded the data. DX, YLY, LJX, XHY, and WX made substantial contributions to the experimental design. WX and DLF authenticated the insects and provided the necessary experimental equipment. All authors proofread the final version and accept responsibility for every aspect of their work.

Ethics statement

The focal species used in this study is not under protection of CITES or wildlife laws in China, its status is not assessed by IUCN. The collecting and handling of the specimen was conducted in accordance with the ‘Regulation on Experimental Animals of Hunnan Province’.

Disclosure statement

The authors disclaim any conflict of interest and take responsibility for the content.

Data availability statement

The genome sequence data that support the findings of this study are openly available in GenBank of NCBI under the accession number OQ354975. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA929694, SRR25319645, and SAMN32968917, respectively.

Additional information

Funding

This work was financially assisted by the National Natural Science Foundation of China [31970450] and [32360134].