The complete chloroplast genome of Porella gracillima Mitt. (Porellaceae) and phylogenetic relationships to other bryophytes

Abstract

Porella gracillima Mitt. (Jungermanniidae, Porellaceae), a bryophyte is widespread in temperate Asia and North America. In Korea, P. gracillima is mainly observed in shaded and dried rocks or tree trunks on mountains. Here, we determined the complete chloroplast (cp) genome sequence of P. gracillima to provide useful genetic information in the phylogenetic relationship, phylogeographic history, and conservation of the species. The complete cp genome of P. gracillima was assembled using NGS Illumina HiSeqX platform. The cp genome was 121,867 bp in length (GC contents, 33.7%) and showed a typical quadripartite structure, consisting of a large single copy (LSC) of 83,406 bp, a small single copy (SSC) of 19,692 bp, and two inverted repeats (IRs) of 9,385 bp. Phylogenetic analysis shows that Porellaceae was a sister group of Radulaceae, which agrees with the findings of the previous phylogenetic studies. Our cp genome data of P. gracillima may contribute to a better understanding of the evolution of the Porella in Porellaceae and will help to infer its molecular identification, thereby providing a guideline for conservation.

Keywords:

• Liverwort
• plastome
• next generation sequencing
• phylogenomic analyses

Author contributions

JP conceived and designed the research. SP collected the plant materials. JL and MP performed the experiments and analyzed the data. JL and MP wrote the draft. JP revised the draft. All authors approved the final version of the manuscript and agreed to be accountable for all aspects of the work.

Ethical statement

Field works have complied with local legislation and appropriate permissions/license were granted while taking samples from protected land. The process and purpose of this experimental research were in line with the rules and regulations of our institute. There are no ethical issues or other conflicts of interest in this study.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The genome data that supported the findings of this study are openly available in GenBank of NCBI at [https://www.ncbi.nlm.nih.gov] under accession no. OQ658673. The associated BioProject, SRA, and Bio-sample numbers are PRJNA990926, SRR25119183 and SAMN36277543 respectively.

Additional information

Funding

This work was supported by a grant from the Honam National Institute of Biological Resources (HNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea [Grant No. HNIBR202101107, HNIIBR202201116].