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Abstract
DNA barcoding constitutes a supplemental genetically based characterization tool for the identification of species. Traditionally, the barcodes are generated with a length of 650 bp using standardized Sanger sequencing, but with the introduction of high-throughput sequencing (HTS) methods new opportunities for sequencing are available. To use HTS for barcode collection and identification, the amplification of shorter fragments is preferred. Reference DNA midi-barcodes of wild bees were produced using the Illumina MiSeq as well as the Sanger method. Although DNA midi-barcodes derived from Illumina were comparatively shorter (418 bp), their sequences were coherent to the morphological assignment of species. The Illumina barcodes proved to be effective and dealt better with some general limitations of DNA barcoding.
Acknowledgements
We wish to thank Felix Gaulhofer for providing samples to our study, Sabine Schoder and Sophie Kratschmer for assisting with the identification of difficult taxa. Furthermore, we wish to thank the national park Donau-Auen for the permission to collect specimens in the year of 2016.
Disclosure statement
No potential conflict of interest was reported by the authors.
