Abstract
Green synthesis of silver nanoparticles using water extract of Bistorta officinalis (BO/Ag-NPs) and evaluation of toxicity and its pro-apoptotic effects. BO/Ag-NPs were synthesized and characterized by DLS, FTIR, XRD, EDX, TEM and FESEM. Then, toxicity and apoptotic effect of BO/Ag-NPs were assessed by MTT, qPCR, flow cytometry and AO/PI methods. Spherical BO/Ag-NPs with a size of 29 nm, PDI of 0.3 and ζ potential of −27.06 mV were synthesized. Medium concentrations of BO/Ag-NPs on MDA-mb231 and HDF cells were reported about 6.1 and >50 µg.ml respectively. Increase the rate of apoptotic cells in AO/PI staining and flow cytometry and upregulation of caspase 3,9 and Bax genes and down regulation of Bcl-2 gene confirmed the activated of intrinsic pathway in BO/Ag-NPs treated cells. Due to the biological effects of BO/Ag-NP, these nanoparticles could be a promising factor in cancer therapy.
Acknowledgment
This study was performed in the Mashhad Branch, Islamic Azad University, Iran and the authors have special thanks to the central lab of this university.
Conflict of interest
No conflict of interest was declared by the authors.
Abbreviations | ||
DLS | = | dynamic light scattering |
FTIR | = | Fourier transform infrared spectroscopy |
XRD | = | x-ray diffraction |
EDX | = | energy dispersive x-ray |
TEM | = | transmission electron microscopy |
FESEM | = | field emission scanning electron microscopy |
MTT | = | ([3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) |
AO/PI | = | acridine orange/propidium iodide |
Bax | = | Bcl-2-associated X protein |
Bcl-2 | = | B-cell lymphoma-2 |
HDF | = | human dermal fibroblasts |
MDA-MB-231 | = | a type of breast cancer cell line |
ζ potential | = | zeta potential |
PDI | = | Polydispersity Index |
FBS | = | fetal bovine serum |
DMEM | = | Dulbecco’s Modified Eagle Medium |
DW | = | distilled water |