Green synthesis, characterization and antimicrobial activity of silver nanoparticles using Ornithogalum narbonense L.

Abstract

Green synthesis of silver nanoparticles (AgNPs) was performed using extracts from Ornithogalum narbonense L. (ONL) as a cost-effective and eco-friendly approach. A surface plasmon resonance peak was observed at 440 nm after 12 h. The intense reddish-brown coloration was observed higher than 1.5 mM AgNO₃ concentrations. Bioactive compounds were found responsible for the reduction, stabilization, and capping of Ag⁺ to AgNPs. X-ray powder diffraction analysis confirmed the face-centered cubic silver crystalline structure. The average spherical-shaped individual particle size was around 8.46 nm, while hydrodynamic size ranged between 7–13 nm and 36–131 nm. The AgNPs have good stability (ζ = –31.15 mV). Energy-dispersive X-ray spectroscopy analysis proved the presence of elemental Ag (6.76%). The antibacterial activity with the zone of inhibition is as follows: P. aeruginosa > E. coli > S.aureus > B. cereus (10.35 ± 0.12 mm > 9.40 ± 0.14 mm > 8.20 ± 0.10 mm > 7.0 ± 0.24 mm). The AgNPs did not show antifungal properties against C. albicans.

HIGHLIGHTS

• The aqueous extract of Ornithogalum Narbonense L was used for the green synthesis of AgNPs.

• XRD, SEM, EDX, FTIR, ζ-potential, DLS and UV-vis characterizations were made.

• The average surface charge and diameter of individual AgNPs were determined as –31.15 mV and 8.46 nm.

• AgNPs acted as an inhibitor toward E.coli, P.aeruginosa, E.faecalis, S.aureus and B.cereus.

Graphical Abstract

Keywords:

• Antimicrobial activity
• green synthesis
• Ornithogalum narbonense L.
• silver nanoparticle
• characterization

Acknowledgments

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Disclosure statement

No potential conflict of interest was reported by the authors.

Authors’ contributions

Dilara Ozturk: conceptualization, writing – original draft methodology, writing – reviewing and editing, investigation, visualization, methodology. Ayse Ozguven: writing – original draft preparation,writing – reviewing and editing, validation, visualization. Vahap Yonten: methodology, writing – original draft preparation, writing – reviewing and editing, visualization. Metin Ertaş: visualization, investigation, writing – original draft preparation, writing – reviewing and editing.

Availability of data and material

Not applicable.

Code availability

Not applicable.

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.