Stimuli-responsive Double Network CMC-based Hydrogel Nanocomposite with enhanced mechanical properties for proliferation and differentiation of PC12

ABSTRACT

A novel double network (DN) hydrogel nanocomposite with good compressive strength has been prepared by using Carboxymethyl Cellulose-g-Acrylic acid-Iron Oxides Nanoparticles (CMC-g-AA-IONs) as the primary network and polyacrylamide as the secondary network. The structure of the synthesized DN hydrogel nanocomposite was investigated by Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), thermogravimetric analysis (TGA), and mechanical strength measurement. The synthesized DN hydrogel showed high mechanical strength (σ of 0.238 MPa), despite the water content of 90%. Its biocompatibility effect on sensitive nerve cells was evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) test. The results showed that the DN hydrogel nanocomposite has no cytotoxicity on these cells and did not cause any disturbance or inhibit their growth and proliferation.

GRAPHICAL ABSTRACT

KEYWORDS:

• Double network hydrogel
• Nanocomposite
• PC12 cell
• Regeneration gene expression
• Viability

Acknowledgments

The authors are deeply grateful to colleagues and laboratory staff of NRC/IUMS for their sincere collaboration and Islamic Azad University Karaj branch

Author's on contributor

Mona Farhadi, Faezeh Shafiei, Fatemeh Shahsavari, Alireza Rezanejad Amirdehi, Tayebeh Amini and Mahsa Bagherpour were involved in experiments and wrote the paper draft. Alireza Rezanejad Amirdehi collected the data and edited the paper, and all the authors read and approved the final manuscript.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Notes on contributors

M. Kurdtabar

M. Kurdtabar, Professor in karaj branch, IAU, majoring in polymer chemistry.

F. Shafiei

F. Shafiei, researcher in karaj branch, IAU, majoring in microbiology.

T. Amini

T. Amini, principal researcher in karaj branch, IAU, majoring inpolymer chemistry.

F. Shahsavari

F. Shahsavari, researcher in karaj branch, IAU, majoring in microbiology.

A. Rezanejad Amirdehi

A. Rezanejad Amirdehi, researcher in karaj branch, IAU, majoring in microbiology.

M. Darestanifarahani

M. Darestanifarahani, researcher in karaj branch, IAU, majoring in microbiology.

M. Bagherpour

M. Bagherpour, researcher in karaj branch, IAU, majoring in microbiology.

M. Farhadi

M. Farhadi, Professor in karaj branch, IAU, majoring in microbiology.