Anhydrosafflor Yellow B alleviates brain injury of acute permanent cerebral ischemia in rats by anti-inflammatory mechanism

Abstract

Anhydrosafflor Yellow B (AHSYB), is an effective ingredient with a high content extracted from safflower. The effect of hydroxysafflor yellow A (HSYA), one of the main compounds in safflower, has been extensively investigated both in experimental study and in clinical practice. However, few studies of AHSYB on inflammation in rats with acute permanent cerebral ischemia was explored. In this study, three doses of AHSYB (1.75, 3.5, 7 mg/kg) were administered to rats. The results showed that AHSYB improved neurological deficit, reduced brain infarct volume, alleviated the brain inflammation and suppressed the elevation of both mRNA and protein levels of heat shock protein 60 (HSP60), toll-like receptor 4 (TLR-4), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). In addition, AHSYB treatment decreased NF-κB p65. All the above effects of AHSYB were more obvious at the dosage of 7 mg/kg. These findings suggest that AHSYB effectively alleviates brain injury followed by acute permanent cerebral ischemia in rats by anti-inflammatory mechanism.

KEYWORDS:

• Anhydrosafflor Yellow B (AHSYB)
• rat
• acute permanent cerebral ischemia
• anti-inflammatory mechanism

Acknowledgements

This work was sponsored by Shanxi Applied Basic Research Project (No. 201901D211538), Science and Technology Innovation Project of Colleges and Universities in Shanxi Province (No. 2019L0734), Open Fund of Shanxi Key Laboratory of Inflammatory Neurodegenerative Diseases in Shanxi Datong University (No. KF2019002), Scientific Research Program of Traditional Chinese Medicine Administration in Shanxi Province (No. 2016ZYYZ02) and Astragalus Resource Industrialization and Industrial Internationalization Cooperative Innovation Center Project of Shanxi Province (No. HQXTCXZX2016-028).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Additional information

Funding

This work was supported by Shanxi Applied Basic Research Project [grant number 201901D211538]; Science and Technology Innovation Project of Colleges and Universities in Shanxi Province [grant number 2019L0734]; Open Fund of Shanxi Key Laboratory of Inflammatory Neurodegenerative Diseases in Shanxi Datong University [grant number KF2019002]; Scientific Research Program of Traditional Chinese Medicine Administration in Shanxi Province [grant number 2016ZYYZ02]; Astragalus Resource Industrialization and Industrial Internationalization Cooperative Innovation Center Project of Shanxi Province [grant number HQXTCXZX2016-028].