Effect of polyherbal drug on oxidative stress and insulin resistance in high-fat diet-induced type 2 diabetic rats

ABSTRACT

Many traditional plants have been used to fight life-threatening diseases such as diabetes, atherosclerosis and cancer. These plants have been shown to possess antioxidant activities, decrease the diabetes nuisance. The effect of 250 mg/kg b.wt of polyherbal drug (PHD) was investigated on high-fat diet-induced diabetic rats and impact of PHD administration was observed. The blood biochemical parameters such as glucose, insulin, hemoglobin, HbA1c, lipid profile and plasma antioxidants were evaluated. The increased levels of blood glucose, cholesterol, triglycerides and low-density lipoprotein cholesterol were noticed. In contrast, the levels of insulin and HDL-cholesterol were diminished. The oral administration of PHD showed a decrease in glucose level, cholesterol, triglycerides and LDL-cholesterol, whereas an increase in insulin level and HDL-cholesterol were recorded. What’s more, the antioxidant enzymes in diabetic control rats showed significantly decreased activities of SOD, catalase, glutathione peroxide activities and reduced glutathione (GSH) compared to treated diabetic rats and the extent of LPO. These results showed that our formulated PHDs have possessed antioxidant potential. The effect produced by the PHD on various parameters was comparable to that of Metformin – a standard anti-diabetic drug.

KEYWORDS:

• Poly herbal drug
• high-fat diet
• antioxidant
• lipid profile

Acknowledgements

The authors gratefully acknowledged the DST-SERB for providing financial support. Award-File No. ECR/2016/001579, New Delhi, India

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The authors gratefully acknowledged the DST-SERB for providing financial support. Award-File No. ECR/2016/001579, New Delhi, India; SERB, India.