The β3 Integrin Gene is Expressed at High Levels in the Major Haematopoietic and Lymphoid Organs, Vascular System, and Skeleton During Mouse Embryo Development

Abstract

Integrins are a family of cell surface molecules that mediate the attachment of cells to the extracellular matrix (ECM). These αβ heterodimers are involved in many biological processes. We used northern blotting and in situhybridization to study the pattern of β3 integrin gene expression during mouse embryogenesis. Northern blotting detected two species of β3 mRNA from 7 to 17 days post coitum (dpc). These transcripts were abundant in the adult testis, kidney, liver, spleen, and heart. In situhybridization experiments detected high levels of β3 in the major haematopoietic and lymphoid organs: yolk sac, liver, and thymus. Moreover, β3 transcripts were also detected in the vascular system, where β3 integrin probably plays a key role in angiogenesis and vasculogenesis. We also detected a hybridization signal in the gut, the bronchioles of the lungs, and the bladder wall. β3 transcripts were also present in the medullary regions of the adrenal glands and in the developing skeleton. Our study shows that β3 gene expression is not restricted to the liver and gut during mouse development. We also detected β3 integrin mRNA in the yolk sac, vessels, lung, bladder, and developing bones. Our data suggest that β3 integrin plays a key role in many important physiological processes like haematopoiesis, angiogenesis, phagocytosis, and bone resorption.

• β3 integrin
• angiogenesis
• haematopoiesis
• osteogenesis
• thymus