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Research Article

Nitric Oxide and Prostacyclin Play a Role in the Regulation of Microvascular Protein and Hydraulic Permeability in Cat Skeletal Muscle

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Pages 233-243 | Published online: 10 Jul 2009
 

Abstract

Objective:To evaluate a possible role of nitric oxide (NO) and prostacyclin in the regulation of basal microvascular protein and hydraulic permeability. Methods:The study was performed on the autoperfused cat calf muscle. Changes in the osmotic reflection coefficient for albumin, calculated from the extended Starling equation, were used as a measure of altered protein permeability, whereas changes in capillary filtration coefficient (CFC) were used as a measure of altered hydraulic permeability. Results:Inhibition of the endogenous NO production with L-nitro-arginine methyl ester given intra-arterially to the muscle decreased the reflection coefficient to 70% of the control (p< 0.05) and increased the CFC by 17% (p< 0.05). The addition of a simultaneous intra-arterial infusion of the NO precursor Larginine restored both the reflection coefficient and the CFC back to control level. Blockade of the endogenous prostacyclin production with tranylcypromine given intra-arterially decreased the reflection coefficient to 72% of the control (p< 0.05) and increased the CFC by 24% (p< 0.05), and they were both restored to control levels by a simultaneous intravenous infusion of a nonvasodilating dose of prostacyclin (1.0 ng/kg per minute). Conclusions:The results support the view that endogenous NO and prostacyclin decrease both protein and hydraulic permeability. Assuming a dynamic endogenous release of these substances, they may act as bidirectional regulators of protein and hydraulic permeability. Microcirculation(2003) 10,233–243. doi:10.1038/sj.mn.7800187

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