Abstract
Cryptococcus neoformansvar. gattiihas regularly been the cause of serious human disease. However, the environmental sources of these infections often remain unclear. During an environmental sampling study, two different strains of C. neoformansvar. gattiiwere isolated from fresh insect frass (order Lepidoptera; family Oecophoridae) in a shallow cavity in the bark of a living Eucalyptus tereticornistree, one molecular type VGI and the other VGII. This is the first published report of the isolation of two different molecular types of C. neoformansvar. gattiifrom a single source, and the third of isolation of molecular type VGII from an environmental source. The potential association with insect frass is consistent with categorising C. neoformansvar. gattiiwithin the Tremellales, containing mycoparasitic fungi.