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Abstract
An HPLC procedure with fluorescence detection has been developed for the quantitation of cisapride in human plasma. Cisapride and the internal standard (azelastine) were extracted with the mixture of cyclohexane-isoamyl alcohol (98:2 v/v) and quantitated using a reverse-phase C18 column. The chromatograms were completely free from interfering peaks, and the retention times of the cisapride and the internal standard were 10 min and 8.5 min, respectively. The method was fully validated in human plasma over a range 0.3–100 ng/ml. The mean intra-day precision and accuracy among triplicate sets of assay were 2.8% and 2.2%, respectively. The mean inter-day precision and accuracy over a period of one week were 4.8% and 5.7%, respectively. Extraction recoveries were 92.3% for the drug and 91.1% for the internal standard, respectively. The lower limit of quantitation of the method was 0.3 ng/ml. The method was simple, reliable and accurate for the quantitation of cisapride in human plasma.