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Abstract
A simple, sensitive and selected fluorimetry for determination of ascorbic acid (AA) is described. The method is based on the reaction of ascorbic acid in alkaline medium with 2,3-diaminonaphthalene without oxidant. The addition of βcyclodextrin (β-CD) greatly enhance the fluorescence of the system. Under optimum conditions, a linear relationship was obtained between the fluorescence intensity and the concentration of ascorbic acid in the range of 0.05–600 μg/ml, the detection limit is 8 ng/ml. In AA-DAN-β-CD system, the inclusion complex is formed. Its inclusion constant is 1.1 × 106L2/mol2.
ACKNOWLEDGMENT
This work was supported by Natural Science Foundations of China and Shandong Province and by visiting scholar Foundation of Key Lab. in University.