SPE-HPLC-DAD DETERMINATION OF TRITON X-100 IN PERFUSABLE SOLUTIONS

A. Medvedovici Faculty of Chemistry, Dept. of Analytical Chemistry, University of Bucharest, Sos. Panduri no. 90, Bucharest, 5, RomaniaCorrespondence[email protected]

V. David Faculty of Chemistry, Dept. of Analytical Chemistry, University of Bucharest, Sos. Panduri no. 90, Bucharest, 5, Romania

F. Tache Faculty of Chemistry, Dept. of Analytical Chemistry, University of Bucharest, Sos. Panduri no. 90, Bucharest, 5, Romania

F. David Research Institute for Chromatography, Kennedypark 20, Kortrijk, B-8500, Belgium

P. Sandra Research Institute for Chromatography, Kennedypark 20, Kortrijk, B-8500, Belgium

Abstract

Determination of sub-ppm Triton concentrations in perfusable solutions using an off-line SPE-HPLC-DAD technique is described. Chromatographic separation is based on a two columns back flush set-up, in order to focus the analyte peak and for eliminating matrix interferences. SPE was mainly used for sample concentration.

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SPE-HPLC-DAD DETERMINATION OF TRITON X-100 IN PERFUSABLE SOLUTIONS

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SPE-HPLC-DAD DETERMINATION OF TRITON X-100 IN PERFUSABLE SOLUTIONS

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