![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
A mitochondria based amperometric biosensor was developed for the determination of L-succinic acid. The biosensor was characterized using hexacyanoferrate (III) as the electrochemical mediator. The electrochemical behaviour of the redox couple hexacyanoferrate (II)/(III) was improved by incorporating octadecylamine into the enzyme modified carbon paste. L-succinic acid was detected at + 0.35 V. Linearity was observed in the range 0.05–0.4 mM of L-succinic acid (sensitivity 0.388 uA/mM, r = 0.996). The detection limit is 0.02 mM. The electrode maintains 60% of its activity after 7 days of use.