MICRODETERMINATION OF PROTEINS BY ENHANCED RESONANCE LIGHT SCATTERING SPECTROSCOPY OF M-ACETYLCHLOROPHOSPHONAZO

Abstract

A determination method of proteins with the limit of determination at nanogram levels is proposed by using a common spectrofluorometer to detect intensity of resonance light scattering (RLS). Under acidic conditions, the reaction between m-acetylchlorophosphonazo (CPA-mA) and proteins enhances the weak light scattering of CPA-mA drastically. The enhanced light scattering intensity is proportional to the concentration of proteins. The linear range of human serum albumin is 0.25–40.0 μg ml⁻¹. The determination of human serum and urine by this method give the results comparable with those by the Coomassie Brilliant Blue G-250 colorimetry (CBB) with relative standard deviations of 1.7–2.54% (n = 6). There is almost no interference from amino acids and most of the ions tested.

Keywords:

• Proteins
• Resonance light scattering
• M-Acetylchlorophosphonazo

ACKNOWLEDGMENTS

This work was supported by the Hebei Doctor Foundation.