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Abstract
Herein we present a study on the application of a thin-layer potentiometry for detection of an interaction of odorants with liposome lipid layer. Three different kinds of liposomes: neutral (phosphatidylcholine + cholesterol), negatively charged (with dicetylphosphate) and positively charged (with sterylamine), were applied for study of the influence of charge on the interaction with odorants. The relative marker ion tetrabutylammonium (TBA+) release from the interior of liposomes upon stimulation by odorants was used as a measure of their interaction with the lipid layer.
ACKNOWLEDGMENTS
The authors acknowledge the State Committee for Scientific Research (KBN) for the financial support of the present work within the Grant no: 5 PO6 G 02615. The authors thank Prof. Yoshio Umezawa (University of Tokyo, Japan) for providing the equipment for the thin-layer potentiometry and Dr. Dariusz Kikut-Ligaj (Academy of Economy Chemistry Department, Poznan, Poland) for calculations by CHEM-X program.