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Abstract
In this paper, a novel flow-injection chemiluminescence (CL) system for the determination of urea is described. It is based on the CL reaction of urea and N-bromosuccinimide (NBS) in alkaline medium. The CL intensity was greatly enhanced in the presence of dichlorofluorescein. The CL intensity was linear with an urea concentration of 6 × 10−6 mol/L ∼ 1 × 10−3 mol/L, the detection limit being 2.7 × 10−6 mol/L (3 s/r). The whole process, including injection, detection and washing, could be completed in 1 min with a relative standard deviation of less than 5%. The proposed method is rapid, simple and suitable automatic and continuous analysis. This method has been applied satisfactorily to the analysis of urea in serum.
ACKNOWLEDGMENT
This study was supported by the National Nature Science Foundation of China (No. 39730160).