CHEMILUMINESCENCE FLOW INJECTION ANALYSIS OF AMOXYCILLIN BY A PERMANGANATE-BASED REACTION

ABSTRACT

A new application of chemiluminescence for the determination of amoxycillin in flow injection mode is presented. The method is based upon the direct chemiluminescence reaction of amoxycillin with KMnO₄ in acidic medium in presence of quinine sulfate. The chemiluminescence intensity was linearly related to the concentration of amoxycillin within 0.05–10 µg mL⁻¹ range with a detection limit (3σ) of 0.02 µg mL⁻¹ and a sample measurement frequency of 90 samples h⁻¹. The relative standard deviation is 2.3% for 1.0 µg mL⁻¹ amoxycillin solution in 11 repeated measurements. The method was successfully applied to the analysis of amoxycillin in capsules.

Keywords:

• Amoxycillin
• Chemiluminescence
• Flow injection
• Potassium permanganate

Acknowledgments